Catalog # NM4361

Nucleoporin p62 (N-terminal region) Antibody

Mouse Monoclonal

Application / Dilution
ELISA1:1000
ICC1:50
WB1:500

Size 100 μl

Species Reactivity Hu, Rt, Ms, Ck

MW 62 kDa

Isotype IgG2b

$360


DATASHEET  

Active transport of proteins and RNA into and out of the nucleus occurs via the nuclear pore complex (NPC). The NPC is formed by a multiprotein complex that includes nucleoporin proteins. Specific nuclear localization sequences found in proteins target proteins for active transport into the nucleus through the NPC. Nucleoporin p62 is the best characterized member of the family of nucleoporins found in the NPC. A tightly associated complex is formed by p62 and two other nucleoporins, p54 and p58. p54 binds to a carboxy-terminal coiled-coil domain of p62 and p58 binds to a dimer of p54. The amino-terminal domain of p62 contains a series of XFXFX repeats and is joined to the coiled-coil domain by a threonine-rich linker segment. The major role of p62 is maintenance of the structural integrity of NPCs.

 

References
Newmeyer, D.D. (1993) Curr Opin Cell Biol. 5(3):395.
Carmo-Fonseca M. et al. (1991) Eur J Cell Biol. 55(1):17-30.

Immunocytochemical labeling of Nucleoporin p62 in paraformaldehyde-fixed and NP40-permeabilized A7r5 cells. The fixed cells were labeled with mouse monoclonal anti-Nucleoporin p62 (N-terminal region) and the antibody was detected using Goat anti-Mouse secondary antibodies conjugated to DyLight® 488.

Western blot image of cell structure markers in NCI-H1915 lung carcinoma cells. The blot was probed with anti-Vimentin intermediate filament protein VM4341 (lane 1), anti-Nucleoporin p62 NM4361 (lane 2), anti-Hsp60 mitochondrial protein HM4381 (lane 3), and anti-Calnexin endoplasmic reticulum protein CM4371 (lane 4).



Clone M436 was generated from a recombinant protein containing amino acid residues in the N-terminal region of human nucleoporin p62. This sequence has high homology to similar regions in rat, mouse, and chicken nucleoporin p62.

*For more information, see UniProt Accession P37198
Mouse monoclonal purified with protein A chromatography is supplied in 100µl phosphate-buffered saline, 50% glycerol, 1 mg/ml BSA, and 0.05% sodium azide. Store at –20°C. Stable for 1 year.

The products are are safely shipped at ambient temperature for both domestic and international shipments. Each product is guaranteed to match the specifications as indicated on the corresponding technical data sheet. Please store at -20C upon arrival for long term storage.

This antibody detects a 62 kDa* protein corresponding to the apparent molecular mass of nucleoporin p62 on SDS-PAGE immunoblots of human HeLa and rat A7r5 cells. In immunocytochemistry, anti-Nucleoporin p62 specifically stains nuclei and nuclear envelope in paraformaldehyde fixed and NP-40 permeabilized cells.

*All molecular weights (MW) are confirmed by comparison to Bio-Rad Rainbow Markers and to western blot mobilities of known proteins with similar MW.

This kit contains: