Catalog # SM1881
Semaphorin-4D (C-terminal region) Antibody
Mouse Monoclonal
| ELISA | 1:2000 |
| ICC | 1:100 |
| WB | 1:1000 |
Size 100 μl
Species Reactivity Hu, Rt, Ms
MW 150 kDa
Isotype IgG1
Plexins are a family of large integral membrane proteins that complex with neuropilins to form semaphorin co-receptors. The extracellular region of plexins contains a semaphorin domain, multiple glycine-rich motifs, and MET-related sequences. The cytoplasmic region contains a Sex/Plexin domain and putative tyrosine phosphorylation sites that mediate signal transduction after activation. In addition, Plexin B1 and B2 contain cleavage sites for subtilisin-like proprotein convertases. These cleavage sites may be important for regulating assembly of heterodimeric receptors or release of the extracellular domain. Plexin-B1 is expressed as a 300 kDa precursor protein that can be cleaved into a 200 kDa a-subunit and 100 kDa b-subunit. This plexin is the receptor for the class IV semaphorin, Sema-4D. Sema-4D activation of Plexin B1 recruits and activates c-Met, which can promote cell survival, migration, and angiogenesis.
References
Basile, J.R. et al. (2006) PNAS 103(24):9017.
Artigiani, S. et al. (2003) J Biol. Chem. 278(12):10094.
Tamagnone, L. et al. (1999) Cell 99:71.
Western blot image of mouse brain lysate. The blot was probed with mouse monoclonal Semaphorin-4D antibody (SM1881) at 1:250 (lane 1), 1:500 (lane 2), and 1:1000 (lane 3).
Immunocytochemical labeling in rat PC12 cells differentiated with NGF. The cells were probed with rabbit polyclonal Plexin B1 (PP1841) and mouse monoclonal Sema-4D (SM1881) antibodies, then the antibodies were detected using appropriate secondary antibody conjugated to DyLight® 594.
*For more information, see UniProt Accession Q92854
The products are are safely shipped at ambient temperature for both domestic and international shipments. Each product is guaranteed to match the specifications as indicated on the corresponding technical data sheet. Please store at -20C upon arrival for long term storage.
*All molecular weights (MW) are confirmed by comparison to Bio-Rad Rainbow Markers and to western blot mobilities of known proteins with similar MW.
This kit contains:
