Catalog # MX3725

Memo (N-terminal region) Peptide

Blocking Peptide

Application / Dilution

Blocking	1:1000
ELISA	50 ng/well

Size 50 μg

During cell migration, actin assembly drives cell membrane protrusion, while microtubules (MTs) extend within protrusions to promote adhesion site turnover. Memo (mediator of ErbB2-driven cell motility) is an effector of the ErbB2 receptor tyrosine kinase involved in breast carcinoma cell migration. This effector may be important for mediating ErbB2-regulated changes in actin and MT dynamics during cell motility. Memo, a 297-amino-acid protein, has homology to class III nonheme iron-dependent dioxygenases, however it has not been shown to display metal binding or enzymatic activity. It has been shown to bind ErbB2 (Tyr-1227) phosphopeptide via its putative enzymatic active site. Memo and PLCγ1 interaction with ErbB2 is essential for HRG-induced chemotaxis. Furthermore, organization of the lamellipodial actin network is coordinated by signaling from Memo to the RhoA–mDia1 pathway localized to the plasma membrane. In addition, Memo may regulate actin dynamics by promoting cofilin depolymerizing and severing of F-actin.

References

Meira, M. et al. (2009) J. Cell Sci. 122:787.
Qiu, C. et al. (2008) J. Biol. Chem. 283:2734.
Zaoui, K. et al. (2008) J. Cell Biol. 183:401.

Western blot analysis of Memo expression in adult mouse heart (lane 1 & 4), mouse C2C12 cells (lane 2 & 5), and rabbit spleen fibroblast cells (lane 3 & 6). The blot was probed with anti-Memo (N-terminal region) (MP3721; lanes 1-6) in the presence (lanes 4-6) or absence (lanes 1-3) of Memo blocking peptide (MX3725).

Memo synthetic peptide corresponding to amino acid residues in the N-terminal region of human Memo. This peptide sequence is highly conserved in rat, mouse, and chicken Memo.

*For more information, see UniProt Accession Q9Y316

Blocking Peptide is supplied in 50µl phosphate-buffered saline and 0.05% sodium azide. Store at –20°C. Stable for 1 year.

The products are are safely shipped at ambient temperature for both domestic and international shipments. Each product is guaranteed to match the specifications as indicated on the corresponding technical data sheet. Please store at -20C upon arrival for long term storage.

The peptide is specifically recognized by Memo (N-terminal) antibody (MP3721) in ELISA, and has been shown to block the reactivity of MP3721 in Western blot. In addition, the peptide is recommended for use in blocking MP3721 reactivity in immunocytochemistry.

*All molecular weights (MW) are confirmed by comparison to Bio-Rad Rainbow Markers and to western blot mobilities of known proteins with similar MW.

This kit contains: